核移植方法和维生素C影响绵羊克隆胚胎体外发育

doi:10.11843/j.issn.0366-6964.2014.09.005

Abstract

Abstract:

The present study was designed to investigate the effects of different nuclear transfer methods and supplementation of culture media with vitamin C on the fusion rate and development of ovine cloned embryos.The abattoir-derived ovaries were collected for materials.After in vitro maturation of oocytes，micro-electrode fusion，zona-free and micro-injection cloning were performed to compare their efficiencies in construction of cloned embryos.Optimal concentration of vitamin C was explored by examining the effects of different concentrations of vitamin C on the parthenogenetic development of oocytes and subsequently applied to in vitro culture of cloned embryos.The results showed that fusion rate obtained from micro-electrode fusion with 25 volts was significantly increased compared with Chamber fusion (P<0.05).Twenty five volts resulted in less dead oocytes than 30 volts in micro-electrode fusion (P<0.05).Zona-free cloning significantly increased the morula rate of cloned embryos (P<0.05)，but failed to increase blastocyst rate significantly (P>0.05).Vitamin C supplementation with the concentration of 50 μmol•L^-1 significantly increased blastocyst rate of ovine parthenogenetic and cloned embryos (P<0.05).Altogether，fusion rate during somatic cell nuclear transfer could be increased by micro-electrode fusion.Blastocyst development of ovine cloned embryos could not be improved by zona-free cloning.Fifty μmol•L^-1 vitamin C was beneficial for in vitro culture of ovine cloned embryos.

CLC Number:

S826
S814.8

MIN Jiang-tao，DU Wei-hua，ZHAO Xue-ming，HAO Hai-sheng，HANG Su-qin，ZHU Hua-bin. Nuclear Transfer Methods and Vitamin C Affect the in vitro Development of Ovine Cloned Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1410-1416.

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Nuclear Transfer Methods and Vitamin C Affect the in vitro Development of Ovine Cloned Embryos

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